9 in. The Spectramax 190 has. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The SpectraMax® 190 Absorbance Plate Reader has a temperature range of 190 - 850 nm, a wavelength accuracy of <±2. For A Higher Degree Of Automation, The Automation Vendor. Photometric Performance SpectraMax 190 Item Description. . 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. no. 5 - 2. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual 1-3 Introduction General Overview The SPECTRAmaxŽ 190 microplate spectrophotometer provides rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. STAKMAX. Glow-type luminescence provides an extended signal time window enabling batch processing of plates in screening assays;Colorimetric changes were measured using a SpectraMax 190 microplate spectrophotometer at 450 nm with a wavelength correction set at 620 nm. Minimize sample volume while retaining sensitivity with the SpectraDrop Micro-Volume Microplate. Find many great new & used options and get the best deals for Molecular Devices SpectraMAX 190 Microplate Reader at the best online prices at eBay! Free shipping for many products!Mini-Bead Beater (BioSpec Products); fluorimeter microplate reader (SpectraMax. Microplate Readers Promega GloMax Plate Reader Multi-Detection. The VersaMax and SpectraMax 190 read 96-well microplates. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. (2012). SPECTRAmax® 190. Multimode Reader Comparison Chart. Thông Số Kỹ Thuật Máy Quang Phổ Định Lượng DNA/RNA SpectraMax Quickdrop - Khoảng bước sóng: 190 -1100 nm - Thể tích nhỏ nhất: 0. Exceptional performanceThe SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. . Figure 1. This instrument is designed to answer the strict requirements of. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. Contact AnytimeLabTraderThe OD values of this chromogenic enzymatic reaction were read on a microplate spectrophotometer (Spectramax 190, Molecular Devices Corporation, Sunnyvale, CA) at 450 nm within 30 min after adding the stop solution. Features. Absorbance equation. SpectraMax M Series Multi-Mode Microplate Readers 7 The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2µL samples with 24- or 64-well plates. Firefly luciferase, a 61 kD monomeric protein, is especially attractive to many researchers because of its high sensitivity, wide linear detection range and extremely low background due to the absence of endogenous luminescent activity in mammalian cells. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. A microplate reader for endpoint, kinetic, and spectral scan assays at UV-visible wavelengths, with temperature control, shaking capabilities, and multichannel optical design. Therefore, the measurement wavelength of the malachite green assay is not. The patented multichannel optical design of the SpectraMax® 190 Absorbance Microplate Reader mimics a dual beam spectrophotometer, measures each sample in the plate. Protein concentrations were calculated using polynomial 2nd analysis from the protein standards. 2) If you wish to regulate the temperature inside the microplate reading cham-. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Analysis of pro-inflammatory macrophage phenotypic response to NAM and RSV. the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. The plate was mixed on an orbital shaker for 15 s and immediately read using a SpectraMax iD3 (Molecular Devices; San Jose, CA, USA) and an integration time of 400 ms. Plate stacker and robot integration The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. 119. Here, we demonstrate several different ways the ABS Plus reader, in combination with the SpectraDrop™ Micro-Volume Microplate and SoftMax® Pro Software, can quantitate double-stranded DNA and bovine serum albumin (BSA. Packed 25 plates per sleeve, 2 sleeves per case. We assessed the expression of pro- and anti-inflammatory genes expressed by macrophages, including. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Page 6 SpectraMax Plus384, 190, 340PC384, and VersaMax. SpectraMax 190 Specifications Technical specifications are subject to change without notice. We used three technical replications for each biological replication and measured absorbance at 595 nm using a SpectraMax 190 spectrophotometer (Molecular Devices, LLC, Sunnyvale, CA). 9 to 190 ng/µL were prepared in TE buffer and pipetted onto the wells (spots) of the SpectraDrop Micro-Volume. Prepare your iD series instrument for shipment. ”1) All parts of the SPECTRAmax 190 Microplate Spectrophotometer are warranted for a period of one (1) year from the original date of delivery. Spectramax 190 Absorbance Microplate reader, included validation report, control software, 190-850 nm, Fully inspected and validated with Warranty. The protein concentration was measured by an enhanced BCA protein assay kit (Beyotime; Nanjing, China). Auction Details Bio + Laboratory Equipment Expo. Temperature control and shaking come as standard. All service plan coverage will end by December 31, 2028. Additional models are available. overnight at room. This robust system is capable of reading 96 wells, each with its own sample beam and reference detector. . All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. The 293T cells were seeded into 6-well plates, using 330,000 cells. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 0 out of 5 $ 4,499-5%. The reaction continued for 7 min and was subsequently stopped by adding 50 μL of 0. SpectraMax® M-Series Readers, SpectraMax® Paradigm® Systems, FlexStation® 3 Systems. The patented multichannel optical design of the SpectraMax 190 Absorbance. Molecular Devices LLC 96 well microplate spectrophotometer spectramax 190 96 Well Microplate Spectrophotometer Spectramax 190, supplied by Molecular Devices LLC, used in various techniques. Two 4" dia, wheels are available: one which accepts six 25mm filters, and one which accepts twelve 12. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Italic and boldface type are used for emphasis. 1. com always has the largest. The VersaMax and SpectraMax 190 read 96-well microplates. The minimum detection limit of the assay was 18. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. You can also contact Technical Support by email. polynomial 2nd analysis from the protein standards. Example: [Drawer]. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes acrossawiderangeofconcentrations. SpectraMax Plus 384 and 340PC 384 readers can accommodate standard. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. These filter wheels are used for a host of applications, including color CCD photography, fluorescence microscopy, and photometry. Wavelength selection Monochromator tunable in 1 nm increments. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. Bioz Stars score: 98/100, based on 1 PubMed citations. This user guide provides detailed instructions on how to operate, maintain, and troubleshoot the device, as well as examples of applications and. Examples: “Press carefully to engage,” “ Do not. . Examples: “Press carefully to engage,” “Do not. Multi-mode Microplate Readers. Brand: Molecular Devices. The following steps provide a quick reminder of the basic operating proce- Overview dures required to read a microplate using the SPECTRAmax 190. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. 01, 0. 3 mmol) was added to a flask containing a 10 mL of methanol solution of HPPH (0. After 1 h of incubation, the absorbance of each well was measured at A450 nm using a SpectraMax microplate reader (SpectraMax 190, Molecular Devices, USA). Highly selective for dsDNA over ssDNA or RNA. Bioz Stars score: 86/100, based on 1 PubMed citations. Widen the range of quantitative experiments with a 190-1000 nm wavelength range. It measures the optical density (OD) of samples in 96-well0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. For very concentrated samples, use a minimum…200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. It measures the optical density (OD) of samples in 96-well 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Microplate Readers Molecular Devices Spectramax iD3. Keywords: spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software,. The absorbance of the reaction mixture was recorded at 593 nm (SpectraMax 190, Molecular devices) after 5 min. spectramax i3x (Danaher Inc) Danaher Inc is a verified supplier. It measures the optical density (OD) of samples in 96-wellLow-volume, high-throughput DNA and protein detection on the SpectraMax ABS Plus Microplate Reader. com. Molecular Devices SpectraMax 340PC384 Microplate Reader $ 4,000. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS control panel are shown in boxed Helvetica type. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Published Dec 16, 2020 | Updated Dec 16, 2020. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. no. Find many great new & used options and get the best deals for Molecular Devices SpectraMax 190 Absorbance Microplate Reader at the best online prices at eBay! Free shipping for many products!An ELISA reader (SpectraMax 190) was used to measure the absorbance of formazan solution at 490 nm. Información para pedidos: Includes current version of SoftMax Pro Software. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers. 7°C (gradient heating design). SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE READING: FluidX: Perception (Vial. SOFTmax PRO. Type: Clear: Add to Cart. . The temperature in the plate chamber is ambient and isothermal. SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Plate chamber design of the SpectraMax category 1 microplate readers. The SpectraMax 190 Microplate Reader is a high-performance spectrophotometer that can measure DNA absorbance across the microplate with a unique quartz fiber optic system and a PathCheck sensor. Luminescence. SpectraMax and VersaMax . It may be named "Pothos". Example: [Drawer]. Gemini™. The UV-vis. 118 Gemini XPS Fluorescence Microplate Reader . ®iD5. Italic and boldface type are used for emphasis. DNA provides the same sensitivity as a spectrophotometer. The SpectraMax 190 reader is the tool for DNA analysis. Wavelength Range: 190 - 850 nm. Multimode Readers Comparison Chart. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentra-tions. The absorbance was read every 30 s for a period of 3 min using a SpectraMax 190 microplate reader. Year Built: N/A. 034 g, 0. Lower limits of detection for ATP determined using the ATPlite . The PathCheck sensor can extend the instrument linearity by a factor of three or more. D. The SpectraMax® 190 UV-Vis Microplate Spectrophotometer from Molecular Devices is ideal for life science applications, especially DNA analysis. Microplate Reader, For Lab. Introduction. Add to Cart Buy. We would like to show you a description here but the site won’t allow us. mg −1 protein using the extinction coefficient of 39. Wavelength accuracy ±1. Dual injectors for kinetic assays. Small volumes (5 μL, 50 mM) of compound solution, dissolved into DMSO, were added to the. Microplate Spectrophotometer. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. Learn more about Services for SpectraMax® 190 Absorbance Plate Reader, Molecular Devices. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 190 – 1100 nm Wavelength accuracy : ±2 nm Stray light <0. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Review date: 12 Jun 2017 | SpectraMax Plus 384 Microplate Reader Get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. . to View Figures. For the GL261 and U87 mouse models, tumor tissue and the contralateral normal brain tissue (non-tumor cells injected hemispheres) was collected as described below and analyzed with the TNFα ELISA kit. 22 × 10 3 M −1 cm −1). We would like to show you a description here but the site won’t allow us. Within the three main categories, microplate readers may differ in the maximum temperature enabled. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. ScanLater Western Blot Detection Cartridge. 384,340PC384,190,ABS,ABS Plus,VersaMax,FlexStation 3 0200-5060 SpectraTest FL1 FluorescenceValidation PlateMOLECULAR DEVICES VERSAMAX SPECTRAMAX 190 INSTALLATION MANUAL Pdf. . EZ-Cytox (10 µL) was added to each of the wells of the plate and incubated for 1 h. Ensure there are three inches between the back of the instrument and any other objects or surfaces so it has proper air flow. These models can no longer be repaired. The technology allows automatic adjustment of good absorbance to a tube equivalent path length of 1 cm, converted to 96 or 384 tubes. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm – 850 nm). . , China). The drawer will open. 304 signal level saturation. The instrument reads any standard cuvette, microcuvette, test tube and 96 well or UV transparent microplate over full 190-1000 nm wavelength range. Molecular Devices SpectraMAX 190 Microplate Reader/Spectrophotometer, Tested. Add to Cart. ®User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected] mg/ml, 80 μl) was incubated at 25°C in the dark for 30 min, then the absorbance recorded at 517 nm (SpectraMax 190 Microplate Reader, USA). , Tokyo, Japan) was added to each well every 24 h. Introduction. Streamline GLP/GMP compliance. Soluble PA fractions (20 μL) were mixed with 100 μL of DMACA reagent on a 96-well plate. Service parts for these models are not being produced and are no longer in stock. Example: [Drawer]. 0 nm. S. The optical density was measured at 540 nm using a SpectraMax 190 microplate reader (Molecular Devices, San Jose, CA, USA). 17247 MOLECULAR DEVICES MICROPLATE SPECTROPHOTOMETER SPECTRAMAX 190. The SpectraMax 190 reader can be integrated with Molecular Devices’ StakMax Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. It measures the optical density (OD) of samples in 96-well Low-volume, high-throughput DNA and protein detection on the SpectraMax ABS Plus Microplate Reader. The SpectraMax® ABS Plus is a compact UV-Vis absorbance microplate reader ideal for these types of quantitative assays. It measures the optical density (OD) of samples in 96-well SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Protein concentrations were calculated using. Protein concentrations were calculated using. Both full length human PI4KIIIB and the truncated PI4KIIIB crystal construct with the c-term were run at 200 nM. 316 grating motor driver faulty. Molecular Devices SpectraMax 190 Microplate Reader. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. I do that by turning the unit off, and turning the wheel. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. The dual-scanning capability can also be used to determine excitation and emission settings for new fluorescent probes. SPECTRAmax 190 Microplate Spectrophotometer Operator’s Manual 2-3 Chapter 2 Setting Up for Stand-Alone Use 1) Place the SPECTRAmax 190 on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- The VersaMax and SpectraMax 190 read 96-well microplates. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected]®Plus384addstheabilitytoread cuvettes. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Three low volume 24-well bottom slides. For more information, please visit us at 190 absorbance plate reader enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. The multi-channel design truly mimics a dual-beam spectrophotometer. 0 out of 5 $ 9,995 $ 9,495. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. With the ability to detect DNA down to 25 ng per well, as well as a limit of calculation to 75 ng per well, the SPECTRAmax 190 microplate spectrophotometer gives you the. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual 1-3 Introduction General Overview The SPECTRAmaxŽ 190 microplate spectrophotometer provides rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. The SpectraMax 190 Microplate Reader's optical system is built around a monochromator, allowing the selection of up to six wavelengths at a time for absorbance detection in the UV-Vis wavelength range (190 nm - 850 nm). Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. The SpectraMax 190 Reader is the tool for DNA analysis. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. View and Download Molecular Devices SPECTRAmax PLUS 384 operator's manual online. The SpectraMax iD3 reader is fully compatible with the SpectraDrop™0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The absorbance was measured at 620 nm using the microplate spectrophotometer (Spectramax 190, Molecular Devices Corporation, Sunnyvale, CA). 96/384-well plate formats. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. Fluorescent Polarization: Solid black plates, as above. Protein content of supernatants was estimated using the BCA reagent. it should turn freely, without any friction. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. 00. 5%T at 220 nm and 340 nm using NaNO2 Absorbance range. The Spectramax 190 has an array of data collection modes, including kinetic, endpoint, and spectral scan modes. Both. 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual iii Labor and Parts To obtain warranty service during the applicable warranty period, you must take the product or de-liver the product properly packaged in the original shipping materials and carton to an authorized Molecular Devices Corporation service facility. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers begining with. Product Description. Example: [Drawer]. Molecular Devices SpectraMax 190 and SoftMax Pro6. Table 1: Categories of heating element design. Molecular Devices technical support is available by phone and e-mail. Tumor cells were plated onto 96-well plates (500 cells/well), and 10 µl of Premix WST-1 Cell Proliferation Assay System (Takara Bio Inc. SPECTRAmax PLUS 384 measuring instruments pdf manual download. Capable of detecting DNA down to 25 ng per well, this model gives users the same sensitivity as conventional systems to produce accurate nucleic acid measurements. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Temperature control and shaking come as standard. to View Figures. ; Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 00. The instrument has a pathlength correction feature. Tumor cells were plated onto 96-well plates (500 cells/well), and 10 µl of Premix WST-1 Cell Proliferation Assay System (Takara Bio Inc. Optimized for fluorescence microplate readers. Molecular Devices SpectraDrop™ Micro-Volume Microplate allows users to read up to 64 samples per plate on SpectraMax® Microplate Readers, with sample volumes as low as 2 µL. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. The optical density (OD) of the samples at 450 nm was measured by a SpectraMax 190, then the LDH activity was further calculated by a relevant formula. Description 1. Multi-mode Microplate Readers. 00 0 bids 2d 20h. Our Price: $6,995. The 200 μL mixture was added into a 96-well plate, and the absorbance was measured at 517 nm by enzyme-labeled instrument (SpectraMax 190, Shanghai, China). The blood was centrifuged at 3000 rpm for 15. SpectraMax 190 and Spect FRET fluorescence, top- le-Mode Detection Fluorescence Sir and bottom-read, cell-based assay, lal injection in both 96- and 384-well lates, excellent sensitivity, Autorinse range, du microp 0+ pre-defined protocols; lac compatible based (visible 405-750 nm)The obtained mixture was reacted in a boiling water bath for 20 min, and then, cooled down immediately. Brand: MOLECULAR DEVICES. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area. Our absorbance plate readers feature our PathCheck Sensor technology and. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-Spectramax Plus 384 Plate Reader. The SpectraTest® ABS1. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. The SpectraMax Plus 384 reader is equipped with an innovation patented by Molecular Devices, the PathCheck® sensor, which measures the depth of a sample in a microplate by optical path length. Standard concentrations ranged from 1x10-11 M to 1x10-6 M. Compounds were tested for inhibition of 3CL pro following a similar protocol with several small modifications. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays. 3 mm. 190-SC - SpectraMax 190 Microplate Reader with 1-Year Service Contract - SpectraMax 190 Absorbance Plate Reader, Molecular Devices - Each מותג: Molecular Devices 36,002. Wavelength bandwidth ≤ 2. • Last day to purchase a service plan for SpectraMax 190 Microplate Reader is December 31, 2027. The absorbance was measured using ELISA analytical instruments SpectraMax 190 (Thermo Fisher Scientific, CA, United States) at a wavelength of 450 nm. See More Information. Colorimetric changes were measured using a SpectraMax 190 microplate spectrophotometer at 450 nm with a wavelength correction set at 620 nm. Turn on the Spectra MAX 190 spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). 1 in stock. 00 . The inter- and intra-assay CV for the SAA analysis were less than 10%. SpectraMax® 340PC384 Microplate Reader for. It offers accurate, precise, and flexible assay development tools, such as PathCheck Sensor Interface, PathCheck ABS1 Absorbance Plate, and SpectraTest ABS1 Absorbance Plate. Three-dimensional Matrigel culture. Add to cart. Robust, high-value microplate readers that can run samples. 0 nm, and a photometric accuracy of <±0. Find regional support. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. Products : SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax 250 (Obsolete), SpectraMax 340 (Obsolete), VersaMax reader How does stray light affect my OD reading? The basic problem caused by stray light (light you don't want, getting into the read) is a falsely low OD seen. Note: Due to. A = 2 - log 10 (%T). The SpectraMax Vertical 1000 uses only the highest-efficacy ams-Osram LEDs with efficiencies up to 4. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. A layer of growth factor-reduced Matrigel was made for 3D cultures . SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual 1-3 Introduction General Overview The SPECTRAmaxŽ 190 microplate spectrophotometer provides rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. After treatment with paramylon or lipopolysaccharide for 24 h, 10 μL of cell counting kit reagents was added to the cells. Molecular Devices Spectramax 190 Absorbance Microplate Reader. , Tokyo, Japan) was added to each well every 24 h. The MIC was determined by the concentration where there was an obvious clear-cut decrease in the absorbance. How do I do this? Note: Check the Applies to section at the bottom of the page to make this is the. Open the catalog to page 1. 7. To provide access to disconnect power from the instrument, maintain a 20 cm to 30 cm (7. 2. Select the Driver tab and click Update Driver. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. The SpectraMax 190 has a multichannel design for 96-well Molecular Devices SpectraMax 190 Temperature range: 4°C above ambient to 45°C Digital controller and display Sold as-is The Molecular Devices SpectraMax 190 can be used to quantify DNA and perform endpoint, kinetic, and spectral scanning analyses. Absorbance was acquired at 450 nM in the microplate reader SpectraMax 190. Description 1. Ltd. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 00. Filter wheels can be shipped preloaded. 13. SpectraMax® 190 absorbance plate reader enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method. 1step assay with SpectraMax microplate readers. The analysis was performed in triplicate and the results were expressed as mg gallic acid equivalent per g of extract (mg GAE/g). 0 nm increments . UV Qualification, using NIST traceable standards, is now available for SpectraMax 190, Plus 384 and the M Series (M2/M3/M4/M5/M5e) Parameters that are checked during the UV Qualification are: Wavelength accuracy. 305 reference level saturation. 5 mM −1 ∙cm −1) in a SpectraMax 190 plate reader (Molecular Devices). In manufacturing DNA/RNA-synthesizers for universities, hospitals and private companies all over the world, the group relies on the SpectraMax 190 Absorbance Microplate Reader. RvTrader. 7°C and the bottom to 36. SpectraMax 190 Absorbance Microplate Reader. ®The SpectraMax Glo Steady-Luc Reporter Assay Kit is designed for optimal performance on microplate readers with high throughput enabled by both the microplate format and extended signal window. On LabX buyers can find. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. . Plus 384 - SpectraMax Plus 384 Microplate Reader - SpectraMax Plus 384 Absorbance Plate Reader, Molecular Devices - Each . Exceptional performance The advanced optical and electronicExplore how the SpectraMax i3 Multi-Mode Platform provides an unlimited breadth of application possibilities, and how it can evolve with your future needs. After incubation, the unbound planktonic cells (120 μl) were. Near bottom. Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $ 3,500. SKU: 17247 Category: Laboratory Equip. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . The Solution Speaking about how OligoMaker uses the SpectraMax 190, Karl Ross-Petersen explains, "We use the SpectraMax 190 as an important step in testing the DNA/RNA. qPCR HBV DNA was extracted as. Ultraviolet transparent multiwell plates from Greiner Highly recommended for measurements of DNA and protein concentrations at 260 nm or 280 nm, respectively By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Standards were plotted using a log-log curve fit in SoftMax Pro Software. The SpectraMax Plus 384 Microplate Spectrophotometer is a versatile and reliable instrument that can measure absorbance in the UV and visible range. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. The SPECTRAmax 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. The absorbance was determined at 450 nm with SpectraMAX-190 ELISA reader (Molecular Devices, Sunnyvale, CA). Apr 21th, 2023Spectramax 190 Microplate ReaderThe SpectraMax 190 Reader Can Be Integrated With Molecular Devices’ StakMax® Microplate Stacker In A Matter Of Minutes And Begin Reading Microplates With Seven Mouse Clicks. Very small footprint suitable for tight space requirements. 00 shipping. SpectraMax Gemini EM & FLEXstation (bottom read) 1. Molecular Devices SOFTmax PRO software is a highly integrated program that can be used to control and collect data from the SPECTRAmax 190. We don't know when or if this item will be back in stock. The corrected absorbance is within 5% of the value. The SpectraMax® 190 Absorbance Microplate Reader is the ideal device for many life science applications, including DNA analysis. 3 Figures from 612 Publications Promega. TheSpectraMax® SpectraMax 190 (Serial number starting with N) SpectraMax 250 SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer to the Obsolete Microplate Reader Models article for additional information. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. When connecting for the first time to either the SpectraMax L reader or StakMax system. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for. Title: SpectraDrop Micro Volume Microplate | Molecular Devices Author: Molecular Devices, LLC Subject:We will match our competitor's price! The SpectraMax® 190 Microplate Reader from Molecular Devices is ideal for UV-Vis life science applications, especially DNA analysis. 1) Select the correct plate and the wells that you want to measure. VC was used as the control substance. SpectraMax ID5 Plate Reader (Molecular Devices) Absorbance. Part # 0112-0085 Rev. The equation that allows one to calculate absorbance from % transmittance is. Reads various kinds of 96-wells plates, and is equipped with shaking function. The ability to detect 16 ng/well of DNA and to quantitate 50 ng of DNA provides the same sensitivity as a spectrophotometer. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. SpectraMax 190 and VersaMax readers can accommodate standard 96-well and strip-well microplates. 16 $ $ 36,002 . Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. 4 mM −1 cm −1 for H 2 O 2. A = Log 10 (I 0 /I). SpectraMax® Paradigm® Multi-Mode Detection Platform.